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Introductory Molecular Genetics: Course Outline

course code: BSMG613

Learning Outcomes

This course covers the brief overview of the cellular & molecular biology, membrane physiology, introduction to molecular medicine and gene therapy, nuclear transplantation, gene therapy for neurological disorders, gene therapy for musculoskeletal disorders and the concept of molecular medicine in physical therapy.

 

This course has been designed to address a more in depth study of biology of inheritance and inheritance patterns. This course focuses on classical Mendelian genetics, the DNA molecule and molecular genetics, and population genetics. The course also covers Human genome and Molecular Pathology.

COURSE CONTENTS

BRIEF REVIEW OF CELLULAR & MOLECULAR BIOLOGY

  • Structure and Functions of Cell, Nucliec Acid, Chromosomes & Proteins

INTRODUCTION TO MOLECULAR MEDICINE AND GENE THERAPY

  • Introduction
  • Genetic Manifestations of Molecular Medicine
  • Gene Therapy and Patterns of Gene Expression
  • Gene Therapy and Molecular Medicine
  • Gene Therapy: Current Basic Science Issues
  • Human Gene Therapy: Current Status and Basic Science

GENE THERAPY FOR NEUROLOGICAL DISORDERS:

  • Introduction
  • Sorting Out the Complexity of the Nervous System
  • What Goes Wrong in Neurological Disorders
  • Neurotophic Factors and Gene Therapy
  • Neural Transplants and Stem Cells
  • Clinical Neurodegenerative Conditions
  • Clinical Trials Testing Genetically Modified Cells and Neurotrophic Factors for Neurodegeneration:
  • Stem Cell Therapy in Spinal Cord Injuries
  • Future Considerations and Issues

GENE THERAPY FOR MUSCULOSKELETAL DISORDERS

  • Bone
    • Introduction:
    • Regulatory Factors in Bone Development and Regeneration:
    • Cells for Gene Therapy Strategies Directed Towards Bone Regeneration
    • In Vivo& Ex Vivo Gene Therapy Strategies for Bone
    • Clinical Trials for Bone Replacement
  • Ligament and Tendon:
    • Introduction

 
    • Ligament and Tendon Growth Factors
    • Cells for Gene Therapy Strategies Directed Towards Ligament Regeneration
    • In Vivo &Ex VivoGene Therapy Strategies to Intact Ligament and Tendon
    • Gene Therapy Strategies for Lacerated Tendon Repair, promote Osseo-Integration of Tendon Grafts
    • Clinical Trials for Ligament and Tendon Replacement:
  • Cartilage:
    • Introduction
    • Growth Factors and Cytokines for Cartilage Repair and Regeneration
    • Cells for Gene Therapy Strategies Directed Towards Cartilage Regeneration
    • Gene Delivery Strategies for Cartilage Repair and Regeneration
    • Dose Dependency Detected with Cartilage Gene Therapies
    • Therapeutic Effects by Transfected Cells on Distal Joints
    • Transfected Xenogenic Cells for Cartilage Repair
    • Cartilage Tissue Engineering and Gene Therapy
  • Intervertebral Disc
    • Introduction
    • The Biology of Intervertebral Disc Degeneration
    • Application of Gene Therapy in Intervertebral Disc
    • In Vivo& Ex Vivo Gene Therapy Strategies to Intervertebral Disc
    • Clinical Trials for Intervertebral Disc
  • Muscles
    • Introduction
    • The Molecular Basis of Myopathies
    • In Vivo& Ex Vivo Gene Therapy Strategies in Myopathies
    • Clinical Trials in Myopathies
    • Gene Therapy: Ethical Issues at the Policy Level

A brief introduction to following topics

  • The chemistry of dna
  • Dna replication and recombination
  • Transcription, translation and protein synthesis
  • Cell-based dna cloning
  • Nucleic acid hybridization assays:
  • Pcr, dna sequencing and in vitro mutagenesis:
  • Organization of the human genome:
  • Human gene expression:
  • Instability of the human genome:
  • Mutation and dna repair:

 
  • Physical and transcript mapping: