Biosafety and Lab Principles: Course content (MLBL-512)

Units of Measurement

• Structure of SI Units
• Standardized Reporting of Laboratory Result Units in Clinical Enzymology

Pathology Laboratory Safety Precautions

• Biological safety
• Chemical safety
• Electrical hazards
• Personal protective equipment
• Occupation safety and health hazards (OSHA)

Specimen collection

• Types of specimens
• Handling of specimens
• Sources of variation in lab results

• • Reagent grade water
• Water purification instruments
• Type1, 2 and 3 water
• Preparation of reagent grade water
• Testing of water quality

Buffers

• Type of buffers
• Measurement of Buffer PH
• Solutions preparation
• Laboratory Glass and Plastic Ware (Use, storage and cleaning)
• Types of Glassware
• Cleaning of Glassware
• Pipettes
• Burettes
• Test Tubes
• Beakers
• Flasks Cylinders

Basic Laboratory Equipment; Operation, calibration, maintenance

• Laboratory Centrifuge
• Balance
• Refrigerators, Freezers And Deep Freezers
• PH meter
• Mixers
• Incubators
• Ovens
• Safety cabinet

Basic Laboratory Procedures Practical

• Calculation of the concentration of the substances determined.
• Preparation of solution
• Preparation of Calibration Curve
• pH measurement
• Use of common lab instruments like balance, water bath, centrifuge etc
• PPE use

First aid

Introduction

• Definition and importance of First Aid
• Contents of first aid box in the lab
• First aid charts

Emergency treatment of cut and bleeding

• Caused by Contaminated glass ware
• Bleeding from a limb
• Bleeding from nose

Emergency treatment of burns

• Heat burns
• Chemical burn of skin
• Chemical injury in eye

Emergency treatment of poisoning

• Swallowing acid or alkali
• Swallowing other poisonous chemicals
• Swallowing of infected material
• Mouth pipetting