Haematology-I: Course content (MLHT-513)

Blood (Cell, plasma, serum)

• Introduction to Haematology
• Haematopoiesis (erythropoiesis, leucopoiesis, thrombopoiesis)
• Haemoglobin synthesis, structure, functions

Etiology, lab diagnosis of the following:

• Anaemias

• Iron deficiency anaemia
• Megaloblastic anaemia
• Aplastic anaemia
• Thalassemia major
• Sideroblastic anaemia
• Anaemia of chronic disorder
• Sickle cell anaemia

Leukocyte disorders

• Leukemias
• Acute Lymphoblastic leukaemia
• Acute Myeloblastic leukaemia
• Chronic Myeloid leukaemia
• Chronic lymphocytic leukaemia
• Lymphomas
• Hodghkins lymphoma
• Non Hodghkins lymphoma

Plasma cell disorders

• Multiple Myeloma

Haemostasis

• Normal Hemostasis

• Hereditary Coagulation Disorders
• Hemophilia A
• Hemophilia B
• Acquired Coagulation Disorders
• Vitamin K Deficiency
• Haemorrhagic disease of the newborn
• Liver Disease
• Disseminated intravascular Coagulation

Practicals

• Prepare and use common anticoagulants
• Operate fully Automated Haematology Analyzer

• Preparation and staining of blood films
• Reticulocyte staining and reticulocyte count
• Bone marrow aspiration and trephine biopsy
• Cytochemical stains

• Haemoglobin studies
• Screening tests for G6PD deficiency
• Sickling test
• Coagulation studies
• Platelet function studies

• Thrombophilia screening
• Cellulose Acetate Membrane Electrophoresis
• Quality control in routine haematology

Hemoglobin

• Equipment /methods of hemoglobin estimation

ESR

• Equipment /methods of ESR estimation
• Hematocrit (Hct) or Packed cell volume (PCV)

Definition and principle of test procedures:

• Micro Hematocrit method
• Macro hematocrit method

Cell Counts

• WBC Count
• Manual - Neubauer chamber technique
• Automated
• Platelet Count

• Manual - Neubauer chamber technique
• Automated
• Total RBC Count
• Manual - Neubauer chamber technique
• Automated
• Total eosinophil Count
• Normal values in adults and infants
• Reticulocyte stain
• Smear preparation for demonstrating reticulocyte
• Source of errors
• Differential leucocyte count (DLC)
• Preparation of blood smear
• Staining of blood smear
• Types of Romanowsky staining (Leishman’s , Giemsa’s, Field’s stain)
• Blood cells morphology (Neutrophils, Lymphocytes, Monocytes , Eosinophils, Basophils)