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Medical Instrumentation: Course content (MLMI-514)

Course description

Clinical Chemistry techniques - Spectral techniques

Light and matter

  • Properties of Light and radiant Energy
  • Interaction of Light with matter

Absorption spectroscopy

  • Radiant energy absorption
  • Beer Lambert law
  • Instrumentation
  • Instrument performance
  • Quality Control checks of spectrometry
  • Reflectance spectrophotometry
  • Recording or spectral spectrophotometry
  • Multiwavelength spectrophotometry

Atomic absorption

  • Principle
  • Instrumentation
  • Sources of Error

Flame Photometry

  • Principle
  • Instrumentation

Fluorometry

  • Principle
  • Instrumentation
  • Limitations
  • Chemiluminescence
  • Electro chemiluminescence
  • Fluorescence polarization

Nephelometry and Turbidimetry

  • Principle
  • Instrumentation
  • Limitations; Nephelometry versus Turbidimetry

Refractivity

  • Principle
  • Application
  • Interference
  • Instrumentation

Electrochemistry

  • Potentiometric Methods
  • Reference electrode
  • Indicator Electrode
  • Ion-selective electrode
  • Care and methodology

Laboratory Automation

  • Laboratory Processes
  • Goals of automation

Course description

Automated laboratory Systems- General considerations

  • Total lab Automation
  • Modular Integrated system
  • Stand-alone Systems
  • Sample processing and Archiving

Concepts of Automation

  • Test Repertoire
  • Random Access
  • Discrete
  • Continous Flow
  • Batch Analyzer
  • Dwell Time
  • Throughput
  • Stat Testing
  • Cost

Measurement of colligative Properties

Colligative Properties

  • Osmosis
  • Osmolality
  • Osmometry
  • Osmolal gap

Clinical uses of Osmometry

  • Plasma Osmolality
  • Urine Osmolality

Principles of Measurement

  • Freezing Point Depression
  • Vapour Pressure depression
  • Osmotic Pressure
  • Boiling point elevation
  • Colloid osmotic pressure

Students should possess sufficient knowledge about following topics:

    • Electrophoresis
    • Chromatography
    • Mass Spectrometry
    • Clinical Enzymology
    • Immunochemical techniques
    • ELISA
    • Electro chemiluminescence
    • FPIA
    • MEIA

Practical: Handling of Common Lab Instruments

    • Spectrophotometers
    • Flame Photometer
    • Ion selective analyser
    • ABG analyser
    • Plasma and urine Osmolality
    • Chemistry auto analyzer