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Transfusion Medicine: Course content (MLTM-513)

Course description

Blood Banking

  • Introduction
  • Requirements for establishment of blood bank
  • Human blood group system (ABO, Rh, and other important blood group systems)
  • Blood grouping: Forward and reverse grouping

          Coombs test

          Blood Transfusion

  • Principal and practice of blood Transfusion
  • Blood Transfusion service at district level
  • Guidelines for the use of blood, appropriate use of blood, quality assurance
  • Autologous blood transfusion practices
  • Objectives of quality assurance in blood transfusion services
  • Standard operating procedure for usage, donation and storage of blood
  • Screening of donor and compatibility testing
  • Safety and procurement of blood components.

          Blood Donation

  • Introduction
  • Voluntary blood donation
  • Criteria for selection of donor
  • Medical history and personal details
  • Self-exclusion
  • Basic lab tests before donating blood
  • Screening for TTI

           Blood Collection

  • Blood Bags
  • Anticoagulants (CPD-A1, CPD-A2)
  • Taking and giving sets in blood transfusion
  • Blood donation procedure
  • Post donation donor care
  • Reaction during and after donation

         Testing donor blood

  • Screening donors blood for infectious agents – HIV, HCV,HBV, Treponema pallidum, malarial parasite

Course description

            Blood donor records

  • Blood donor record book
  • Recording Results
  • Blood Donor Card

Storage and Transport

  • Storage of blood (RCC) / Components
  • Changes in blood after storage
  • Refrigerated centrifuge
  • Lay out of a blood bank refrigerator
  • Transportation of Blood components

              Maintenance of blood bank records

  • Blood bank temperature sheet
  • Blood bank stock sheet
  • Blood transfusion request form

Compatibility testing a. Purpose

  • Single tube compatibility techniques using AHG reagent
  • Emergency compatibility testing
  • Difficulties in cross matching
  • Labeling and issuing cross matched blood

Blood components

        Collection of blood for components preparation

  • Red cell concentrates (RCC)
  • Platelet concentrates (PCC)
  • Fresh Frozen Plasma (FFP)

Blood Transfusion           

Reactions Practicals

  • ABO Blood grouping (Forward and Reverse)
  • Coombs Test (Direct coombs and indirect coombs)
  • Rh Grouping (Detection of Du antigen)
  • Rh antibody screening and Rh antibody titer
  • Cross match (Minor and major cross match)
  • Visit to AFIT