Cell As a Fundamental Unit of Life
- Cell as unit of life
- Compare the eukaryotic and prokaryotic cell
- Concept of cell cycle, mitosis and meiosis and cell death
- Control of cell proliferation and differentiation
- The structure and function of chromosomes
Cytoskeleton
- Function of cytoskeleton
- Major types of protein filaments that form cytoskeleton.
- Mechanism of cyclic contractions of muscles. ( Skeletal and cardiac)
- Mutations in the different genes
Structure of cell membrane
Lipid bilayer (different type of lipid molecules in cell membrane.
General properties of
- Lipid bilayer
- Its fluidity
- Asymmetry
- Glycolipids
Membrane proteins
- Association with lipid bilayer ( lipid anchoring)
- Transmembrane proteins
- Glycosylation of proteins
- Solubilization and purification of membrane proteins
Compartmentalization of cell
- Cell organelles -structure and function of membrane-bounded organelles; protein targeting; the endoplasmic reticulum; the secretory pathway and vesicular transport
- Movement of proteins between compartments
- Movement of molecules between nucleus and cytosol
- Endocytosis
- Exocytosis
- molecular basis of diseases resulting from defects in these processes.
Transport Mechanism
- Introduction uniport, symport, antiport, simple diffusion Transport and channels
- Facilitated Transport
- Types of ATPases + Active transport sec active
- ABC transporter & Diseases related to it.
- Interactions between cells in multicellular systems: extracellular matrix and connective tissue; epithelia and cell junctions.
Methods to study cell Structure
- Principal: methods used to study cells and their molecular components
- How to separate cells of different types from tissues
- How to grow cells outside the body
- How to disrupt cells and lsolate their organelles and constitutional macromolecules in pure form
- Techniques to determine protein structure.
- What is cell lines, uses and advantages