Biochemistry
• Introduction to Biochemistry
• Importance of Biochemistry for Medical Lab Technologist
• Cell:
Structure, function and types of cell (Biochemical Aspects)
• Cell Membrane Structure and function
• Cytoplasm and cytoplasmic organelles
• Cell Receptors & Signal Molecules
Body Fluids
• Structure and properties of Water
• Acids, Bases and Salts 18
• Concept of pH & pK
• Buffers, their mechanism of action
• Body buffer
Biomolecules
• Define biomolecules.
• Types and importance of biomolecules Proteins Biochemistry
• Amino acids occurring in protein molecules:
• Standard amino acids and their classification
• Non-standard amino acids and their importance
• Peptide bonds and their properties
• Protein classification: Simple, Conjugated and Derived proteins
• Proteins three dimensional Structure and their properties
• Primary, Secondary, Tertiary & Quaternary Structures of Proteins
• Sanger’s, Edman’s and DNA sequencing methods
• Laboratory methods for protein detection and isolation
• Electrophoresis, Isoelectric focusing, Chromatography, ELISA
Enzymes
• Introduction and properties of Enzymes
• Mechanism of action of enzymes in reaction
• Chemical nature of enzymes
• Co-enzymes and their actions
• Classification of enzymes
• Enzymes kinematics: MM equation and Line Weaver-Burke Plot
• Factors affecting enzyme activity
• Regulation & Inhibition of Enzyme activity & enzymes inhibitors
• Clinical Diagnostic Enzymology
• Special diagnostic tests using enzymes
• Enzymes, enzyme inhibitors, enzymes activator, enzymes inducers as medicines
• Disease caused by enzyme deficiency
Carbohydrates
• Definition, Classification, Biochemical Functions & Significance of Carbohydrates 19
• Structure & Properties of Monosaccharaides
• Isomerism, optical isomerism and Chirality in monosaccharaides
• Chemical properties of monosaccharaides
• Structure & Properties of Disaccharides
• Structure & Properties of Polysaccharides
• Heteropolysaccharides: GAGs, Glycoconjugates and mucilages
Lipids
• Classification of Lipids
• Fatty Acids: Chemistry
• Classification occurrence & Function
• Structure & Properties of Simple Lipids
• Waxes and their importance
• Structure & Properties of Triacylglycerols
• Structure & Properties of Compound or Complex Lipids
• Steroids and sterols
• Terpenes
• Cholesterol: Chemistry, Functions & Clinical Significance
Vitamins
• Sources, RDA, Biochemical Functions & Clinical Significance of Fat Soluble Vitamins
• Sources, RDA, Biochemical Functions & Clinical Significance of Water Soluble
• Vitamins.
This program will provide the knowledge and practical skill to the medical lab trainees, who would be able to:
• Learn and apply theory and techniques in the medical pathology laboratory.
• Avoid problems pertaining to collecting, transporting, handling and conducting tests on laboratory samples.
• Confidently and correctly carry out all the bench work required for diagnostic tests in various discipline of pathology.
• Demonstrate proper handling and preventative maintenance of instruments.
• Convey timely laboratory results to the clinicians.
• Perform and monitor quality control in the laboratory.
• Assist the consultants in research.
• Demonstrate ethical behavior and decision making
