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Molecular Biology and Genetics I-II (MGBL-301) (MBGL-312): Course Outline

Molecular Biology and Genetics seek to understand how the molecules that make up cells determine the behavior of living things. Biologists use molecular and genetic tools to study the function of those molecules in the complex milieu of the living cell.

Course Contents - (MGBL-312)

• Over view of regulation of gene expression. Regulatory sequences, transcription
factors,
• Regulation of gene expression in prokaryotes (operon systems), eukaryotic gene
regulation,
• Transcriptional control of gene expression (RNA polymerases, regulatory
sequences, activators and repressors of transcription; genetic switches, transcription
initiation, molecular mechanisms of transcription repression and activation, regulation
of transcription factor activity)
• Post-transcriptional controls (processing and transport of pre-mRNA, cytoplasmic
mechanisms of post-transcriptional control, processing of rRNA and tRNA)
• Bacterial protein synthesis and regulation
• Eukaryotic protein synthesis and regulation; post translational modification; non
coding RNAs
Genetics
Multiple alleles, multiple factor hypothesis and use of binomial theorem
Linkages and crossing over and their calculations
Gene mapping,
Sex linkages, Sex determination, sex linked inheritance.
• Gene and environment
Practicals
• Gel Electrophoresis
• Blotting
• Transformation
• PCR

Course Objectives

Aims and Objectives of the Program:
This program will provide the knowledge and practical skill to the medical lab trainees, who
would be able to:
• Learn and apply theory and techniques in the medical pathology laboratory.
• Avoid problems pertaining to collecting, transporting, handling and conducting
tests on laboratory samples.
• Confidently and correctly carry out all the bench work required for diagnostic tests in
various discipline of pathology.
• Demonstrate proper handling and preventative maintenance of instruments.
• Convey timely laboratory results to the clinicians.
• Perform and monitor quality control in the laboratory.
• Assist the consultants in research.
• Demonstrate ethical behavior and decision making.
 

Course Content - (MGBL-301)

Course Contents
• Introduction to molecular biology and history
• Chemical and physical properties of nucleic acids
• The structure and function of DNA, RNA and Proteins
• Prokaryotic and Eukaryotic genes and genome, DNA packaging in chromatin and regulation of chromatin structure
• Structure of chromosomes
• Evolution of genomes (nuclear and organelle genome)
• Central dogma of molecular biology; DNA replication; Transcription; Translation
• Mutations in DNA
• DNA repair including description of types of DNA damage, various DNA
polymerases and different types of repair
• DNA recombination; Transposable DNA elements
Genetics
• Introduction to Genetics
• Introduction to Genetics
• Mendelian Genetics (Mendel laws of assortment and segregation)
• Heredity and Variation
• Gene interaction-genotypic and phenotypic ration