Biochemistry (DVM): Course Outline (101)

Goal:  The course aims to provide the basic understanding of the core principles and to understand the relationship of Biochemistry to health and disease

Objective: The students will be able to define, describe and comprehend the basic biochemical reactions pathways, cycles and their relationship with one another and homeostasis of the organisms.

Theory: The pH & its importance, Buffer, Henderson-Hasselbalch equation, Carbohydrates: Introduction to carbohydrates, Classification of carbohydrates, Glycosidic linkages & Glycosides, Isomers, Optical activity & rotations, Chemical properties of monosaccharides, structure and functions of important monosaccharide, Homopoly saccharide, Heteropoly saccharides, Proteins: Introduction to Proteins, Classification of proteins, Structure and Classification of amino acids, Amphoteric properties of amino acids, Concept of Isoelectric pH, Peptide Linkage, Primary, Secondary, Tertiary and Quaternary structure of proteins, Enzymes; General Characteristics and classification of enzyme, Enzyme Kinetics Lipids: Introduction to Lipids, structure & classification of fatty acids, nomenclature of fatty acids, Physical properties of fatty acids and triglycerides, Chemical Properties of fatty acids and triglycerides, Sterols and cholesterol, Prostaglandin and their physical role, Carbohydrate metabolism: Glycolysis, regulation, energy production, Kreb’s Cycle, regulation, energy production, Electron Transport Chain, Pentose phosphate shunt, Gluconeogenesis, Protein metabolism: Degradation of Proteins and amino acids, Urea cycle and its importance, Urea toxicity, Regulation of urea cycle, Bioenergetics of protein metabolism, Lipid metabolism: Beta oxidation of even and odd chain fatty acids, Biosynthesis of fatty acids, Biosynthesis of triglycerides, Biosynthesis of Cholesterol, Biosynthesis of Ketone bodies, DNA metabolism: Biosynthesis and Degradation of nucleic acids, Comparative features among animal species of particular relevance, metabolism of mono-gastric and poly-gastric animals, and comparison of metabolism of mono and poly-gastric animals.

Practical: Preparation of solutions: Preparation of Percentage, Molar and Normal solutions, Preparation of Buffer, Carbohydrate Analysis: Group Identification Test, Identification of mono, di and polysaccharides, Identification of reducing sugars, Identification of ketose sugar and their confirmation by phenylhydrazine test, Identification scheme for carbohydrates, Estimation of glucose in blood sample by glucose oxidase method, Estimation of amino acids by spectrophotometry, Biuret Test and Ninhydrin Test, Precipitation tests of proteins: Precipitation of casein at isoelectric point, salt saturation tests, Serum protein fractionation by electrophoresis, Separation of the colour pigments of Berseem/ alfaalfa by paper chromatography, Identification of lipids, Salkowiski’s test

• Equal opportunity
• Intellectual honesty
• Regularity and punctuality
• Adherence to deadlines
• Fairness
• Conformity to discipline