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Microbiology & Sterilization - (MBSL-601) (MIT): Course Content

Microbiology is the study of microorganisms, which are microscopic living organisms like bacteria, viruses, fungi, and protozoa. Sterilization, on the other hand, is the complete removal or destruction of all forms of microbial life, including their spore

Course Outline

FUNDAMENTALS OF MICROBIOLOGY
 Microorganisms and their respective place in the living world.
 Differentiation between eukaryotic and prokaryotic cells,
 Historical development of Microbiology and its scope.
MICROSCOPY
 An outline of the principles and applications of light and electron microscope
 Morphology, arrangement and detailed anatomy of bacterial cell
 Bacterial taxonomy and nomenclature
 Basis of classification of bacteria
 Growth, nutrition (physical and nutritional requirement and nutritional types;
sources of energy, C, N, H, O, S, P, trace elements, growth factors)
 Reproduction
GENERAL METHODS OF STUDYING MICROORGANISMS
Cultivation, isolation, purification and characterization
Control of microorganisms by physical and chemical methods Chemotherapeutic agents and antibiotics
Modes of action of antibiotics on microorganisms
Basic properties of fungi
STERILIZATION AND DISINFECTION
Physical methods– (sunlight, Drying, Dry heat, moist heat, filtration, Radiation,
Ultrasonic and sonic vibration)
Chemical- (Alcohols, Aldehydes, Dyes, Halogens, Phenols, Gases)
 

Course Objectives

Course Learning Objectives:
1. Understand the fundamental principles of microbiology, including bacteriology, virology, and immunology, to grasp the essential concepts underlying microbial life and interactions. (C2)
2. Describe the key features of bacterial, viral, fungal, and protozoal structure, growth, physiology, and behavior, enabling a comprehensive understanding of microbial
characteristics and functions. (C2)
3. Explain the genetic and metabolic strategies employed by microorganisms, and discuss their ecological roles in various environments, emphasizing their impact on human health and
ecosystems. (C2)
4. Analyze the need of microbiological techniques effectively, including aseptic techniques, staining methods, and microbial identification procedures, used in diagnostics. (C3)

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