Microbiology: Course Outline
The branch of science that deals with microorganisms
- Defense mechanisms of the body.
- Microbial mechanisms of invasion and virulence.
- Differentiation between sterilization and disinfection.
- Appropriate methods of disinfection and sterilization for the following:
- Spillage: blood and body fluids such as sputum, vomitus,stool, urine,
- Equipment and items:
- Critical items: surgical instruments, sutures, bandages, surgical drapes
- Semi critical items: endoscopes, laryngoscopes, vaginal speculum, proctoscope, thermometer, nasal and ear specula and spatula
- Non critical items: bed linen, floor, blood pressure apparatus, bedrail
- Principles of aseptic techniques for venepuncture, urinary catheterisation, wound dressing, suturing and lumbar puncture.
- Healthcare associated infections and basic concepts of infection control including standard precautions
- General principles of the following serological tests Precipitation (VDRL) and agglutination (Latex particle) and
- Haemagglutination TPHA test
- Immunofluorescent FTA
- ELISA Hepatitis (A, B, C,D,E,) Rubella, Cytomegalovirus (CMV) and Human Immunodeficiency Virus (HIV)
- Western blot for HIV
- ICT Hepatitis Band C.
- Culture reports,
- Serological reports and
- Microscopic reports of Gram and AFB stain.
- Laboratory diagnosis of infectious diseases: Principles of proper sample collection and submission of specimens for laboratory investigations with due precautions.