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Microbiology: Course Outline

The branch of science that deals with microorganisms

Course Outline


  1. Defense mechanisms of the body.
  2. Microbial mechanisms of invasion and virulence.
  3. Differentiation between sterilization and disinfection.
  4. Appropriate methods of disinfection and sterilization for the following:
  5. Spillage: blood and body fluids such as sputum, vomitus,stool, urine,
  6. Equipment and items:
  7. Critical items: surgical instruments, sutures, bandages, surgical drapes
  8. Semi critical items: endoscopes, laryngoscopes, vaginal speculum, proctoscope, thermometer, nasal and ear specula and spatula
  9. Non critical items: bed linen, floor, blood pressure apparatus, bedrail
  10. Principles of aseptic techniques for venepuncture, urinary catheterisation, wound dressing, suturing and lumbar puncture.
  11. Healthcare associated infections and basic concepts of infection control including standard precautions
  12. General principles of the following serological tests Precipitation (VDRL) and agglutination (Latex particle) and
  13. Haemagglutination TPHA test
  14. Immunofluorescent FTA
  15. ELISA Hepatitis (A, B, C,D,E,) Rubella, Cytomegalovirus (CMV) and Human Immunodeficiency Virus (HIV)
  16. Western blot for HIV
  17. ICT Hepatitis Band C.
  18. Interpretation:
  19. Culture reports,
  20. Serological reports and
  21. Microscopic reports of Gram and AFB stain.
  22. Laboratory diagnosis of infectious diseases: Principles of proper sample collection and submission of specimens for laboratory investigations with due precautions.

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