Microbiology (MBBS): Course Outline

MICROBIOLOGY

1. Defense mechanisms of the body.
2. Microbial mechanisms of invasion and virulence.
3. Differentiation between sterilization and disinfection.
4. Appropriate methods of disinfection and sterilization for the following:
5. Spillage: blood and body fluids such as sputum, vomitus,stool, urine,
6. Equipment and items:
7. Critical items: surgical instruments, sutures, bandages, surgical drapes
8. Semi critical items: endoscopes, laryngoscopes, vaginal speculum, proctoscope, thermometer, nasal and ear specula and spatula
9. Non critical items: bed linen, floor, blood pressure apparatus, bedrail
10. Principles of aseptic techniques for venepuncture, urinary catheterisation, wound dressing, suturing and lumbar puncture.
11. Healthcare associated infections and basic concepts of infection control including standard precautions
12. General principles of the following serological tests Precipitation (VDRL) and agglutination (Latex particle) and
13. Haemagglutination TPHA test
14. Immunofluorescent FTA
15. ELISA Hepatitis (A, B, C,D,E,) Rubella, Cytomegalovirus (CMV) and Human Immunodeficiency Virus (HIV)
16. Western blot for HIV
17. ICT Hepatitis Band C.
18. Interpretation:
19. Culture reports,
20. Serological reports and
21. Microscopic reports of Gram and AFB stain.
22. Laboratory diagnosis of infectious diseases: Principles of proper sample collection and submission of specimens for laboratory investigations with due precautions.
23. Classification of microorganisms: General characteristics and taxonomy of Bacteria, Rickettsia, Chlamydia, Viruses and Fungi.
24.  Definition of communicable endemic, epidemic and pandemic diseases, carriers, pathogens, opportunists, commensals and colonizers.
25.  Micro-organisms responsible for infection of the following organ systems:
26.  Central nervous system
27. Respiratory system
28. Gastrointestinal system
29. Genital infections
30. Urinary system
31. Infections of bone and joints
32.  Systemic infections
33. Infection of the skin
34. Hepatobiliary and pancreatic infections
35. Zoonosis
36. Pathogenesis, treatment, epidemiology, prevention and control of following organisms.
37.  Bacteria: Staphylococcus aureus, Streptococcus,Beta hemolytic streptococcus group A & B, Diphtheria sp, Bordetella sp, Bacillus anthracis, Clostridia (perfringens, botulinum, difficile, tetani), Actinomyces israeli, Nocardia asteroides, Neisseria (meningitides and gonorrhoeae), Gardenella vaginalis, Haemophilus influenzae, Mycobacterium tuberculosis and leprae, Escherichia coli, Klebsiella, Proteus, Salmonella, Shigella, Yersinia pestis, Pseudomonas, Vibrio cholera, Vibrio parahemolyticus, Campylobacter jejuni, Helicobacter pylori, Legionella, Lycoplasma pneumoniae, Chlamydia, Treponema pallidium, Leptospira, Rickettsia sp.
38. Viruses: Enterovirus, Mumps, Herpes, Adenovirus, Measles, Influenza, Parainfluenza, Rhinovirus, Respiratory Syncitial Virus (RSV), Hepatitis A, B, C, D, E, Rotavirus, Astrovirus, Cytomegalo Virus (CMV), Ebstein Barr Virus (EBV), Rubella, Chicken pox, Arbovirus, HIV, Rabies.
39. Fungus: Cryptococcus neoformis