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Biochemistry for Biomedical: Course Outline (BM-243)

The branch of science concerned with the chemical and physico-chemical processes and substances which occur within living organisms.

Course Objective

i) To provide an introduction to the basic concepts of biochemistry.
ii) To learn about the structure, classification and functions of protein and enzymes.
iii) To learn about the lipids, vitamins & carbohydrates.

Course Outline

Introduction to Biochemistry: Colloidal state, buffer, pH, significance of pH Henderson equation, surface tension, viscosity, osmosis, diffusion, concept of chromatographic techniques (TLC, paper chromatography, GLC column chromatography etc.) carbohydrates, amino acids, nucleic acids, proteins, vitamins, enzymes, hormones & signaling agents, 

Metabolism of Carbohydrates, Lipids and Proteins: Carbohydrate derivatives, optical activity, polarimetry, glycogenesis, gluconeogenesis, glycolysis, tricarboxylic acid cycle, hexose monophosphate shunt. Effects of hormones on carbohydrate metabolism. Chemistry and Metabolism of Lipids, Proteins

Title of Practicals

1. (a). General test for carbohydrates
    (b). General test for polysaccharides
2. Determine the pH of different given samples
3. To study the cell fragility
4. Estimation of Blood glucose level
5. To detect essential amino acids color reaction test.
6. To determine the protein in the given solution
7. Isolation of casein from milk.
8. Isolation of glycogen from liver.
9. To find out viscosity of the given solution
10. To study colorimeter
11. Estimation of plasma cholesterol level.
12. Effect of temperature on enzyme activity.
13. Separation of amino acids by chromatography
14. Study of nucleic acid (Software)
15 Preparation of solutions (Buffers)

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