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Basic Lab principles, Lab Safety & first aid: Course Outline

course code: RLT604

Course content

Units of Measurement

  • Structure of SI Units 
  • Standardized Reporting of Laboratory Result 
  • Units in Clinical EnzymologY

Pathology Laboratory Safety Precautions

  • Biological safety 
  • Chemical safety 
  • Electrical hazards 
  • Personal protective equipment 
  • Occupation safety and health hazards (OSHA)

Specimen collection

  • Types of specimens 
  • Handling of specimens 
  • Sources of variation in lab results

Reagent grade water

  • Water purification instruments 
  • Type1, 2,and 3 water 
  • Preparation of reagent grade water 
  • Testing of water quality

Buffers

  • Type of buffers 
  • Measurement of Buffer PH

Solutions preparation

Laboratory Glass and Plastic Ware (Use, storage and cleaning)

  • Types of Glassware 
  • Cleaning of Glassware 
  • Pipettes 
  • Burettes Page 11 of 106 
  • Test Tubes 
  • Beakers 
  • Flasks Cylinders

Basic Laboratory Equipment; Operation, calibration, maintenance

  • Laboratory Centrifuge 
  • Balance 
  • Refrigerators, Freezers And Deep Freezers 
  • pH meter 
  • Mixers 
  • Incubators 
  • Ovens 
  • Safety cabinet

Basic Laboratory Procedures Practical

  • Calculation of the concentration of the substances determined. 
  • Preparation of solution 
  • Preparation of Calibration Curve 
  • pH measurement 
  • Use of common lab instruments like balance, water bath, centrifuge etc 
  • PPE use

Course content

First aid

Introduction

  • Definition and importance of First Aid 
  • Contents of first aid box in the lab 
  • First aid charts

Emergency treatment of cut and bleeding

  • Caused by Contaminated glass ware 
  • Bleeding from a limb 
  • Bleeding from nose

Emergency treatment of burns

  • Heat burns 
  • Chemical burn of skin 
  • Chemical injury in eye

Emergency treatment of poisoning

  • Swallowing acid or alkali 
  • Swallowing other poisonous chemicals 
  • Swallowing of infected material 
  • Mouth pipetting

Emergency treatment of Fainting 

Emergency management of Electric shock 

Emergency resuscitation when a person stops breathing

  • Mouth to mouth breathing

First aid in cases where the heart stops beating

Code of safety for the labs

  • Formulating a code of safe laboratory practice 
  • Enforcing a code of safe laboratory practices