Molecular genetics is concerned with the analysis of human nucleic acids as they relate to disease. It is intimately tied to molecular and cell biology and the central paradigm of molecular biology that genes code for proteins. The important topics covered in this subject are nucleic acids – DNA, RNA and protein synthesis in cells. Molecular biology is a branch of biology that is also closely related to other sub-disciplines like biochemistry, cell biology, genetics, and genomics.
1. Melt curves analysis
2. Nucleic acid labeling
3. In situ hybridization (ISH)
4. Restriction fragment length polymorphism (RFLP)
5. Epigenetic modification detection
6. Array technology (e.g., bead, microarray)
7. Multiplex ligation-dependent probe amplification (MLPA)
8. Mass spectrophotometry (e.g., MALDI-TOF)
9. Multi-locus sequence typing (MLST)
Introduction to Human genetics
Chromosomal structure and function
Chromosomal anomalies and human disorders
Organization and expression of human genome
Cancer genetics
Functional genomics, Proteomics and Bioinformatics
Over view of microbial genetics
Molecular science
Nucleic acid chemistry
Sugars
Bases
Chemical structure
Associated proteins
Mutations
Basic Molecular Theory
Replication
Transcription
Exons, introns, and splicing
Translation
Chromosome structure
Extra chromosomal structure (e.g., phage,
plasmid, mitochondrial)
Protein structure
Biochemical Reagents
Polymerase enzymes
DNA / RNA
Endo and exonuclease enzymes
Reverse transcriptase
DNA ligase
Assay development and design
Nucleic Acid Amplification
Polymerase chain reaction (PCR)
Oligonucleotide design and preparation
Reaction optimization
Sequencing
Sanger sequencing (e.g., chain terminators)
Next-generation sequencing
Other (e.g., pyro sequencing)
b. Nucleic Acid Isolation
Automated methods
Manual methods
c. Separation and Detection
Electrophoresis
Gel (including agarose and acrylamide)
Capillary
2. Blotting and probing procedures (including washing and stringency)
3. Probe hybridization
4. Nucleic acid purification
5. Probe structure (e.g., TaqMan®, FRET, simple, beacon, Scorpions®)
1. Mendelian Genetics
2. Human pedigree analysis
3. Analysis of various stages of cell division
4. Karyotyping
Molecular Biology gives you in-depth knowledge of biological and/or medicinal processes through the investigation of the underlying molecular mechanisms. You will gain an understanding of chemical and molecular processes that occur in and between cells