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Basic molecular biology and genetics: Course Outlines RLT- 639

Molecular biology and genetics

Molecular genetics is concerned with the analysis of human nucleic acids as they relate to disease. It is intimately tied to molecular and cell biology and the central paradigm of molecular biology that genes code for proteins. The important topics covered in this subject are nucleic acids – DNA, RNA and protein synthesis in cells. Molecular biology is a branch of biology that is also closely related to other sub-disciplines like biochemistry, cell biology, genetics, and genomics.

Other Techniques

1. Melt curves analysis

2. Nucleic acid labeling

3. In situ hybridization (ISH)

4. Restriction fragment length polymorphism (RFLP)

5. Epigenetic modification detection

6. Array technology (e.g., bead, microarray)

7. Multiplex ligation-dependent probe amplification (MLPA)

8. Mass spectrophotometry (e.g., MALDI-TOF)

9. Multi-locus sequence typing (MLST)

Genetics

 Introduction to Human genetics
 Chromosomal structure and function
 Chromosomal anomalies and human disorders
 Organization and expression of human genome
 Cancer genetics
 Functional genomics, Proteomics and Bioinformatics
 Over view of microbial genetics

 

Course Content

Molecular science
   Nucleic acid chemistry
 Sugars
 Bases
 Chemical structure
 Associated proteins
 Mutations
  Basic Molecular Theory

 Replication
 Transcription
 Exons, introns, and splicing
 Translation
 Chromosome structure
 Extra chromosomal structure (e.g., phage,
 plasmid, mitochondrial)
 Protein structure
 Biochemical Reagents
 Polymerase enzymes
DNA / RNA
 Endo and exonuclease enzymes
 Reverse transcriptase
 DNA ligase
 Assay development and design

Nucleic Acid Amplification
 Polymerase chain reaction (PCR)
 Oligonucleotide design and preparation
 Reaction optimization

Sequencing
 Sanger sequencing (e.g., chain terminators)
 Next-generation sequencing
 Other (e.g., pyro sequencing)

Molecular Techniques

b. Nucleic Acid Isolation
Automated methods
Manual methods
c. Separation and Detection
Electrophoresis
Gel (including agarose and acrylamide)
Capillary
2. Blotting and probing procedures (including washing and stringency)
3. Probe hybridization
4. Nucleic acid purification
5. Probe structure (e.g., TaqMan®, FRET, simple, beacon, Scorpions®)

Practical

1. Mendelian Genetics
2. Human pedigree analysis
3. Analysis of various stages of cell division
4. Karyotyping

Outcomes

Molecular Biology gives you in-depth knowledge of biological and/or medicinal processes through the investigation of the underlying molecular mechanisms. You will gain an understanding of chemical and molecular processes that occur in and between cells