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Histopathology Instruments / Techniques: Course Outline

Course Code RLT-629

Course content

1. Automation in Histopathology
2. Specimens for Histopathology
Basic Techniques

  • Biopsy Specimen collection including cytology specimens
  • Tissue fixation (Rationale, Types & Techniques
  • Gross cutting (Observation. To handle common surgical pathology
  • Specimens including importance of slicing large specimens.
  • Salient features to be described as per recommended guidelines).
  • Tissue processing (Steps & Rationale)
  • Special precaution for tissue to be sent for (electron microscopy, special biopsies, immune
  • fluorescence etc)
  • Decalcification (Methods
  • Basic Molecular Biology Techniques & FISH testing for Her-2- neu /common solid tumor testing
  • Specimen, slides and block storage protocols


  • Routine H & E staining
  • Special stains
  • Immuno histochemical staining, requirements and principles

Basic Hematology

Hematology is the study of blood and the disorders related to it. Human blood consists of blood cells and plasma. Blood has many functions, including transporting oxygen and nutrients to tissues, removing waste materials (e.g., carbon dioxide, urea), regulating body temperature, and carrying cells responsible for coagulation and immune response.