Introduction, scope and importance of biochemistry; Brief introduction of prokaryotic and eukaryotic cells; Bio-macromolecules: composition and organization; Energy and Principles of bioenergetics; Water: Properties of water, acid/base properties, dissociation of water and pH value, pH buffering capacity, transportation mechanisms across bio-membranes and osmosis, Proteins: Amino acids - structure, nomenclature, classification, Primary structure of proteins - peptide bond, sequencing, synthesis, Secondary structure - α-helices, β-sheets, Three dimensional structure of proteins, methods for protein structural determination - X-ray, NMR and homology modeling, tertiary and quaternary structures of proteins, protein denaturation, Methods for purifying and studying proteins; Enzymes: functions, mode of action, specificity and inhibition, classification and nomenclature, factors affecting enzymes activity; Introduction to carbohydrates (Glycobiology): biosynthesis, metabolism, glycolysis, Kerbs cycle, Mitochondrial electron transport chain and ATP synthesis; Lipids: introduction, lipogenesis, lipids and lipoproteins in relation to lipid storage diseases, sterol and steroids; Overview of nucleic acids.
Model visualization of prokaryotic and eukaryotic cells; Solution preparation; Preparation of different buffers and their pH adjustment; Activity of different enzymes like amylase in saliva; Enzyme purification; DNA extraction; Gel electrophoresis; Determination of amino acid profile using HPLC/Amino acid analyzer; Energy estimation through Bomb Calorimeter.
