Immunology and Serology: Course content (MLIS-513)

Course contents:

• Historical background, general concepts of the immune system, innate and adaptive immunity; active and passive immunity; primary and secondary immune response. Cell and organs of immune system, Phagocytosis, Innate immunity and cell mediated immunity
• Antigens and immunogens: Properties,foreignness, molecular size, heterogeneity, B and T cell epitopes; T dependent and T independent antigens.
• Antibodies: Historical perspective of antibody structure; structure, function and properties of the antibodies; different classes, subclasses and biological activities of antibodies; concepts of antibody diversity, isotype, allotype,
• Introduction of hybridoma technology, monoclonal antibodies, polyclonal antibody Mechanism of humoral and cell mediated immune response.
• Introduction of Major Histocompatibility Complex, organization of MHC and inheritance in humans; Antigen presenting cells, antigen processing and presentation Complement system and complement fixation test.
• Complement system
• Introduction of transplant immunology, graft rejection, tissue typing for kidney and bone marrow transplant, Laboratory test for transplant. 
• Autoimmune disorders, pathogenesis, organ specific and systemic autoimmune disorders and its markers such parietal cell antibody, anti sperm antibody, lupus anticoagulants, anti mitochondrial antibody, ANA, ds DNA, HLA-B27, ASMA, anti CCP 
• Immunological disorders: primary and secondary immunodeficiency, SCID, AIDS, Tumour, types of tumours, Various Tumour Markers, their significance and method of estimation.
• Vaccines, classification and applications, Active and passive immunization, Immunoprophylaxis schedule in neonates, children and in pregnancy 
• Hypersensitivity and its types Introduction to Allergy and its laboratory test

 Practicals:

• agglutination,
• precipitation,
• ELISA,
• RIA,
• Immunofluorescence
• RA test
• WIDAL test
• RPR test.
• CRP test.
• Skin sensitivity test
• HCV test
• HBS test
• Cell culture
• Bacterial agglutination test
• Agar gel precipitation test
• Complement fixation test
• Haemagglutination and Haemagglutination inhibition test
• Delayed hypersensitivity test

• Fluorescent antibody techniques
• Immunoelectrophoresis