Molecular Biology and Genetics – II: Course content (MLMB-523)

At the end of the course Students will be able to Know about the gene regulation and gene expression and understand the processes of transcription and translation

• Over view of regulation of gene expression. Regulatory sequences, transcription factors.
• Regulation of gene expression in prokaryotes (operon systems), eukaryotic gene regulation.
• Transcriptional control of gene expression (RNA polymerases, regulatory           sequences, activators and repressors of transcription; genetic switches, transcription initiation, molecular mechanisms of transcription repression and activation, regulation of transcription factor activity)
• Post-transcriptional controls (processing and transport of pre-mRNA, cytoplasmic mechanisms of post-transcriptional control, processing of rRNA and tRNA)
• Bacterial protein synthesis and regulation
• Eukaryotic protein synthesis and regulation; post translational modification; non coding RNAs

• Multiple alleles, multiple factor hypothesis and use of binomial theorem
• Linkages and crossing over and their calculations
• Gene mapping,
• Sex linkages, Sex determination, sex linked inheritance.
• Gene and environment

• Gel Electrophoresis
• Blotting
• Transformation
• PCR