Microscopic Examination of Un-stained bacteria
- Visibility of unstained organism
- Hanging drop preparation
- Detecting motility using MIU medium
Examination of Specimens by Dark field microscopy
Examination of microorganism in stained preparation.
- The following stained techniques will be learnt & practiced
- Gram staining
- Ziehl-Neelsen staining for Mycobacterium tuberculosis
- ZN staining for M. leprae
- Auramine (fluorochrame) staining
- Methylene blue
- Wayson’s
- Albert
- Giemsa
- HISS
- Acridine orange (Fluorochrome)
- Type of staining basic stains and Acidic stains
- Mordants
- Decolorization
- Technique of staining smears
- Quality control
Smear making
Culture of micro organism
- Microbial growth requirement
- Common ingredients of culture media
Type and selection of culture media
- Basic, enriched, enrichment, selective, differential & Transport
Solid, Semisolid & fluid media with examples
Inoculation of culture media
- Aseptic technique
- Making a wire loop
- Inoculation of culture media in petri dishes, slops, as stabs and fluid media
Incubation of cultures
- Temperature of incubation
- Humidity
- Gaseous atmosphere
- Culturing of anaerobes
- Anaerobic jars using hydrogen from a cylinders
- Gas generating kits method
- Use of copper coated steel wool to remove oxygen
- Use of dithionite in petri dishes
- Use of reducing agents in culture media
- Culturing in Carbon-dioxide
- Control of culture media
- Control of stains & regents
Culture/isolation
- Media
- Techniques
- Incubation
- Atmosphere
- Duration
- Temperature
