Skip to Main Content

Advanced Biochemical Techniques (BIOL-707) Mphil: Course Outline

Course Outline

COURSE OBJECTIVES

On successful completion of the course students will be able to:

  • Use biological terminology and advanced concepts and techniques in experimental biology
  • Apply scientific method in advanced experimental design
  • To learn major advanced clinical research techniques employed in the diagnosis of various diseases 
  • To develop and enhance practical skills in the postgraduate students for the revolutionary progress in human genomics

COURSE CONTENT

  • RT-PCR and RFLP 
  • qPCR
  • Blotting Techniques such as Southern, northern, western, dotblot etc. 
  • Flowcytometry
  • Karyotyping
  • Fluorescent in situ hybridization (FISH)
  • Chromogenic in situ hybridization (CISH)
  • Analysis of amniotic fluids and various DNA tests. 
  • Maternal serum testing
  • Use of Chorionic Villous Sampling for the detection of chromosomal and genetic disorders