COURSE CONTENT:
Molecules of Life:
- Analyze the structure and function of biomolecules in living organisms.
- Evaluate the role of macromolecules in cellular processes and disease.
Extraction of Nucleic Acids:
- Develop protocols for the extraction and purification of nucleic acids from various sample types.
- Evaluate the quality and quantity of extracted nucleic acids using spectrophotometric and electrophoretic techniques.
Polymerase Chain Reaction (PCR):
- Evaluate the feasibility and design of PCR assays for specific applications.
- Critically analyze the optimization and troubleshooting of PCR reactions.
Gel Electrophoresis of DNA:
- Analyze and interpret agarose and polyacrylamide gel electrophoresis results.
- Develop strategies for optimizing and troubleshooting gel electrophoresis protocols.
Quantitative PCR:
- Develop and evaluate qPCR assays for quantification of nucleic acids.
- Analyze and interpret qPCR results for various applications.
Identification of Point Mutations:
- Develop and evaluate strategies for detecting and analyzing point mutations in nucleic acids.
- Critically analyze the interpretation and significance of point mutations in disease.
Gene Expression Studies:
- Develop and evaluate methods for analyzing gene expression patterns in various sample types.
- Critically analyze the interpretation and significance of gene expression data in disease.
- Restriction Fragment Length Polymorphism (RFLP):
- Develop and evaluate RFLP assays for detecting genetic variations.
- Analyze and interpret RFLP results for various applications.
- Short Tandem Repeat (STR) Analysis:
- Develop and evaluate STR analysis protocols for forensic and genetic applications.
- Analyze and interpret STR analysis results for various applications.
- Study of Micro RNA:
- Develop and evaluate methods for studying miRNA expression and function.
- Critically analyze the role of miRNAs in disease and therapeutic applications.
- New Developments in PCR:
- Analyze and evaluate the latest advancements in PCR technology and applications.
- Critically evaluate the potential impact of new PCR technologies on research and clinical applications.
- Genomic Sequencing:
- Develop and evaluate protocols for high-throughput genomic sequencing.
- Analyze and interpret genomic sequencing data for various applications.
- Next Generation Sequencing:
- Develop and evaluate methods for analyzing large-scale genomic sequencing data.
- Critically analyze the interpretation and significance of NGS data in disease.
- Sequence Variant Nomenclature:
- Develop and evaluate strategies for standardizing sequence variant nomenclature.
- Analyze and interpret sequence variant data using standard nomenclature.
- Quality Control in Diagnostic PCR:
- Develop and evaluate quality control measures for diagnostic PCR assays.
- Analyze and interpret quality control data to ensure accurate and reliable diagnostic results.
- Setting-up a PCR Lab:
- Develop and evaluate strategies for setting up and maintaining a PCR laboratory.
- Analyze and interpret safety and quality control measures for PCR laboratory operations.
- Basic Cytogenetics:
- Develop and evaluate methods for analyzing chromosomal abnormalities using cytogenetics.
- Critically analyze the interpretation and significance of cytogenetic data in disease.
- Fluorescent in Situ Hybridization (FISH):
- Develop and evaluate FISH protocols for visualizing chromosomal abnormalities.
- Analyze and interpret FISH results for various applications.
- PCR Applications in Inherited Disorders:
- Develop and evaluate PCR-based methods for diagnosing inherited disorders.
- Analyze and interpret PCR data for various inherited disorders.
- PCR Applications in Neoplastic Disorders:
- Develop and evaluate PCR-based methods for diagnosing neoplastic disorders.
- Analyze and interpret PCR data for various neoplastic disorders.
- PCR Applications in Infectious Disorders:
- Evaluate the strengths and limitations of PCR-based methods for detecting and diagnosing infectious diseases.
- Analyze the role of PCR in identifying antibiotic-resistant strains of bacteria
- Develop and optimize PCR assays for detecting specific infectious agents
- Clinical Applications of NGS:
- Evaluate the strengths and limitations of different NGS technologies for clinical applications such as cancer genomics, pharmacogenomics, and rare disease diagnosis.
- Analyze the ethical, legal, and social implications of using NGS in clinical settings, including issues related to informed consent, privacy, and genetic discrimination.
- Forensic DNA Testing:
- Evaluate the reliability and accuracy of forensic DNA testing methods, including STR analysis, mtDNA analysis, and SNP profiling.
- Develop and validate DNA-based methods for identifying suspects, victims, and missing persons in forensic investigations.
- Study of Donor Chimerism:
- Evaluate the methods and technologies used to study donor Chimerism in transplant recipients, including PCR-based assays, SNP profiling, and NGS.
- Analyze the clinical implications of donor Chimerism, its impact on transplant success, graft-versus-host disease.
- Develop and optimize assays for detecting and quantifying donor Chimerism in transplant recipients.
- Pre-Implantation Genetic Diagnosis:
- Evaluate the ethical, legal, and social implications of pre-implantation genetic diagnosis (PGD) for various conditions, including genetic disorders and chromosomal abnormalities.
- Analyze the different types of PGD techniques available, including PCR-based methods, FISH, and NGS.
