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Advances in Cell and Molecular Biology: Course Outline (MOLL-703)

This course provide knowledge about the recent developments in cell biology and advance knowledge of life processes at molecular level.

Course Outline

Foundations of Biomedical Sciences, Understanding and scientific basis of Molecular Aspects of Cell and Gene Therapy, Biology of stem cells and gene delivery and the scientific basis of cell and gene therapy, Causes and genetic basis of diseases that can be treated by stem cells and gene therapy. Techniques and methods involved in the research and application of cell and gene therapy such as basic aspects of Aseptic technique, Cell culture, Cytometric analysis and sorting, Immunocytochemistry, fluorescence and confocal microscopy. Understanding of the basic mechanisms of development, Model organisms, Embryology, Cell fate determination, Differentiation, Pattern formation, Polarity, Cell migration, and cell interactions. Engineering approaches to cell-related phenomena important to cell/tissue engineering, Receptor/ligand binding. Trafficking/signaling processes. Application to cell proliferation, adhesion and motility. Cell-matrix interactions. An awareness of ethical and regulatory aspects, historical, current and future clinical trials of cell and gene therapy.

Introduction to molecular biology and history; Chemical and physical properties of nucleic acids, The structure and function of DNA, RNA and Proteins; prokaryotic and Eukaryotic genes and genome, DNA packaging in chromatin and regulation of chromatin structure; Structure of chromosomes; Evolution of genomes (nuclear and organelle genome); Central dogma of molecular biology; DNA replication; Transcription; Translation; DNA repair including description of types of DNA damage, various DNA polymerases and different types of repair; DNA recombination; Transposable DNA elements. Over view of regulation of gene expression. Regulatory sequences, transcription factors, Regulation of gene expression in prokaryotes (operon systems), eukaryotic gene regulation, Transcriptional control of gene expression (RNA polymerases, regulatory sequences, activators and repressors of transcription; genetic switches, transcription initiation, molecular mechanisms of transcription repression and activation, regulation of transcription factor activity); post-transcriptional controls (processing and transport of pre-mRNA, cytoplasmic mechanisms of post-transcriptional control, processing of rRNA and tRNA); Bacterial protein synthesis and regulation; Eukaryotic protein synthesis and regulation; post translational modification; non coding RNAs.

 

  1. Microscopy and staining techniques
  2. Study of prokaryotic and eukaryotic cells
  3. Critical review and evaluation of  scientific literature.
  4. Students attend Stem Cell research Institute/conference/workshop/ online presentations. Critical analysis and written summaries of the conferences and oral presentation.

Text Books: Cell

Text Books