The course covers general and specialized topics in enzymology and specifically designed for biochemistry students. It is expected that students must have successfully undertake and passed BCH 312- Enzymology which serves as prerequisite before registering for the advanced enzymology. The earlier part of the course will deal with subjects such as steady state kinetics, Michealis – Menten equation and the transient kinetic methods. General topic in enzyme catalyzed reaction such as enzyme catalysis, mechanisms of action, binding energy, enzyme assay, stereochemistry, enzyme /substrate interaction, allosteric interactions and other regulatory enzyme will be adequately cover. The course will be concluded by introducing students to other practical and applied subject; molecular model of allosterism, enzyme reconstitution, criteria for determining purity of enzyme, genetic engineering, protein engineering and biotechnology.
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