Molecular Biology and Genetics-I: Course content (MLMB-513)

At the end of the course students will be able to recognize the comparison of prokaryotic and eukaryotic genes and genomes and know about molecular genetics and how DNA and RNA work

• Introduction to molecular biology and history          
• Chemical and physical properties of nucleic acids
• The structure and function of DNA, RNA and Proteins
• Prokaryotic and Eukaryotic genes and genome, DNA packaging in chromatin and regulation of chromatin structure
• Structure of chromosomes
• Evolution of genomes (nuclear and organelle genome)
• Central dogma of molecular biology; DNA replication; Transcription; Translation
• Mutations in DNA
• DNA repair including description of types of DNA damage, various DNA polymerases and different types of repair
• DNA recombination; Transposable DNA elements

Genetics

• Introduction to Genetics
• Introduction to Genetics
• Mendelian Genetics (Mendel laws of assortment and segregation)
• Heredity and Variation
• Gene interaction-genotypic and phenotypic ration

Practicals

• Preparation of solutions of different concentrations
• Extraction and purification of nucleic acids from different sources (bacteria, blood, plants, animal tissues) etc.
• Quantification of DNA by spectrophotometer
• Gel Electrophoresis
• Qualitative and quantitative analysis of nucleic acids